Indirect Somatic Embryogenesis: An Efficient and Genetically Reliable Clonal Propagation System for Ananas comosus L. Merr. Hybrid “MD2”

The objective of this study was to establish an efficient—direct or indirect—regeneration system for pineapple (Ananas comosus L.) plants, with a high rate multiplication and that would preserve the genetic identity donor genotype (Hybrid ‘MD2’) in regenerated plants. Ten treatments, different concentrations 2,4-Dichlorophenoxyacetic (2,4-D) Picloram (P), absence presence 6-Benzylaminopurine (BAP), were used vitro morphogenesis induction, as well histological molecular techniques, order characterize morphogenic responses induced. Significant differences between treatments tested, induce callus buds, assessed by Kruskal Wallis method Mann–Whitney U-tests. Different regeneration routes identified, showing potential species. medium containing 2 mg L−1 2,4-D BAP, where indirect somatic embryogenesis occurred, selected most efficient treatment, average 120 embryos per explant, differing significantly from rest treatments. It also demonstrated plants preserved genotype, which represents degree confidence application A. comusus clonal propagation.